LASER-INDUCED FLUORESCENCE ANALYSIS DEVICE AND SEP

专利名称：LASER-INDUCED FLUORESCENCE ANALYSIS

DEVICE AND SEPARATION APPARATUSCOMPRISING SAME

发明人：COUDERC, FRANCOIS,NERTZ, MICHEL申请号：EP02791506.5申请日：20020722公开号：EP1410000A1公开日：20040421

摘要：Capillary tube device for analysis of laser-induced fluorescence of substances insolution for detecting and dosing trace substances, has a tube wall comprised of amaterial with a refractive index below that of the water in a channel or above that of boththe water in the channel and the air outside it. The capillary tube device has a tube (2)with a channel (11) containing a solution with at least one substance which produces alaser-induced fluorescent reaction. The tube is made of material which is transparent toan exciting light, projected locally into part (16) of the channel at an angle greater than60 deg to the longitudinal axis (A) of the channel. An optical collector (7) collects theresulting fluorescent light from the channel and is coupled to an optical measuringdevice (8). The resulting signals are treated in (9) to produce an analysis of the solution.Near the second end of the tube is a conical, ellipsoidal or parabolic internal wall withone side turned towards the first end of the tube. The tube has a larger internal sectionthan the outlet pipe and includes a fluorescence detection cell adjacent to the junction(4) connecting the separation unit outlet to the second end of the tube so the detectioncell contains the excitation zone for the substance(s). The second end of the tube has an

internal shoulder for the outlet pipe to butt against, of the same internal diameter as theoutlet pipe. A hollow T connector (5) is placed so the top of the T connects the waveguide of the collector and the end of the tube. The foot of the T forms a side branch (30)for circulation of the solution and/or transport of the substance(s) in and out of the tube.This side branch connects the first end of the tube to a reservoir which has electrodesplaced across the wall. The solution conducts electricity and the electrodes are

connected so there is a difference in potential between the ends of the tube to migratethe substance(s) through the tube by electrophoresis. A second hollow T connector atthe other end of the tube can connect straight through to a second collector andprovides a side branch. The tube then contains a second conical, ellipsoidal or parabolicinternal wall near the first end, with one side turned towards the second end of the tube.The internal walls are coated with a reflective material which is resistant to oxidation. Thetube has an internal diameter of up to 200 micro m for using capillary electrophoresisand up to 1000 micro m for using HPLC. The exciting light is projected by a light source(12) at the side of the tube and optical means (14) between the light source and thechannel to match the cross section of the light beam to the internal diameter of thechannel. The external wall of the tube is coated with a reflective material except near theexcitation zone of the detection cell which must be transparent to the beam (F). Theexcitation beam is elliptical, with the major axis perpendicular to the longitudinal axis ofthe capillary tube and the minor axis parallel to the longitudinal axis of the tube. AnIndependent claim is also included for a device for separation by chromatographyincluding an analysis device as described above.

申请人：PICOMETRICS

地址：701, chemin d'Embeoune,Montlaur 31450 Montgiscard FR

国籍：FR

代理机构：Abello, Michel

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